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lasergene primerselect software version 7 1 0  (DNASTAR)


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    Structured Review

    DNASTAR lasergene primerselect software version 7 1 0
    Lasergene Primerselect Software Version 7 1 0, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 10685 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lasergene+primerselect+software+version+7+1+0/Lasergene/us10842794-621-46-47
    Average 99 stars, based on 10685 article reviews
    lasergene primerselect software version 7 1 0 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Software:

    Article Title: Cyclooxygenase-2 Modulates Glycosaminoglycan Production in the Skin During Salt Overload.
    Article Snippet: .. Primers were designed by Lasergene PrimerSelect software version 7.1.0 (DNAStar, RRID:SCR_016295) based on nucleotide sequences from the National Center for Biotechnology Information’s nucleotide database (Table 1). .. Enzyme-Linked Immunosorbent Assay The quantity of PGE2 and HA in the cell culture media was determined according to the manufacturer’s instructions by using both the High Sensitivity PGE2 ELISA Kit (Abcam, Cat. No. ab133055, Cambridge, UK) and the Hyaluronic Acid ELISA Kit (R&D Systems, Cat. No. HYAL0), respectively.

    Article Title: RAAS inhibitors directly reduce diabetes‐induced renal fibrosis via growth factor inhibition
    Article Snippet: 500 ng Tgfb1, Pdgfb, Ctgf, Mmp2, Timp1, Timp2 mRNA from rat kidney samples and 500 ng TGFB1, PDGFB, and CTGF mRNA from human proximal tubular epithelial cells were reverse-transcribed using First Strand cDNA Synthesis Kit for RT-PCR (Thermo Fisher Scientific, Waltham, MA, USA). .. Aforementioned mRNA expressions were determined in triplicate of 1 μL cDNA sample by qPCR using 10 μL SYBR Green I Master enzyme mix (Roche Diagnostics, Mannheim, Germany) and 10 pmol μL -1 of each specific primers (Invitrogen, Budapest, Hungary) designed by Lasergene PrimerSelect software version 7.1.0 (DNASTAR, RRID:SCR_016295) based on nucleotide sequences from National Center for Biotechnology Information’s nucleotide database (primer sequences: Table 1). .. Results were analysed by LightCycler 480 software version 1.5.0 (Roche Diagnostics, This article is protected by copyright.

    Article Title: Use of Sigma-1 receptor agonist compounds
    Article Snippet: One hundred fifty nanograms each of FN1, COL1A1 and MMP2 mRNA from HTM-5 cells were reverse-transcribed using a First Strand cDNA Synthesis Kit for RT-PCR (Thermo Fisher Scientific, Waltham, Mass., USA). .. The expression of the mRNA was determined in triplicate with 1 μL cDNA samples obtained by qPCR using 10 μL SYBR Green I Master enzyme mix (Roche Diagnostics, Mannheim, Germany) and 10 pmol μL-1 of each specific primer (Invitrogen, Carlsbad, Calif., USA), following sequences designed by Lasergene PrimerSelect software version 7.1.0 (DNASTAR, Medison, Wis., USA) based on nucleotide sequences from National Center for Biotechnology Information's nucleotide database. .. Results were analysed by LightCycler 480 software version 1.5.0 (Roche Diagnostics, Indianapolis, Ind., USA).

    Article Title: The role of sigma-1 receptor and brain-derived neurotrophic factor in the development of diabetes and comorbid depression in streptozotocin-induced diabetic rats.
    Article Snippet: Rationale Depression is highly prevalent in diabetes (DM).. Brain-derived neurotrophic factor (BDNF) which is mainly regulated by the endoplasmic reticulum chaperon sigma-1 receptor (S1R) plays a relevant role in the development of depression.. Objectives We studied the dose-dependent efficacy of S1R agonist fluvoxamine (FLU) in the prevention of DMinduced depression and investigated the significance of the S1R-BDNF pathway.

    Real-time Polymerase Chain Reaction:

    Article Title: RAAS inhibitors directly reduce diabetes‐induced renal fibrosis via growth factor inhibition
    Article Snippet: 500 ng Tgfb1, Pdgfb, Ctgf, Mmp2, Timp1, Timp2 mRNA from rat kidney samples and 500 ng TGFB1, PDGFB, and CTGF mRNA from human proximal tubular epithelial cells were reverse-transcribed using First Strand cDNA Synthesis Kit for RT-PCR (Thermo Fisher Scientific, Waltham, MA, USA). .. Aforementioned mRNA expressions were determined in triplicate of 1 μL cDNA sample by qPCR using 10 μL SYBR Green I Master enzyme mix (Roche Diagnostics, Mannheim, Germany) and 10 pmol μL -1 of each specific primers (Invitrogen, Budapest, Hungary) designed by Lasergene PrimerSelect software version 7.1.0 (DNASTAR, RRID:SCR_016295) based on nucleotide sequences from National Center for Biotechnology Information’s nucleotide database (primer sequences: Table 1). .. Results were analysed by LightCycler 480 software version 1.5.0 (Roche Diagnostics, This article is protected by copyright.

    Article Title: Use of Sigma-1 receptor agonist compounds
    Article Snippet: One hundred fifty nanograms each of FN1, COL1A1 and MMP2 mRNA from HTM-5 cells were reverse-transcribed using a First Strand cDNA Synthesis Kit for RT-PCR (Thermo Fisher Scientific, Waltham, Mass., USA). .. The expression of the mRNA was determined in triplicate with 1 μL cDNA samples obtained by qPCR using 10 μL SYBR Green I Master enzyme mix (Roche Diagnostics, Mannheim, Germany) and 10 pmol μL-1 of each specific primer (Invitrogen, Carlsbad, Calif., USA), following sequences designed by Lasergene PrimerSelect software version 7.1.0 (DNASTAR, Medison, Wis., USA) based on nucleotide sequences from National Center for Biotechnology Information's nucleotide database. .. Results were analysed by LightCycler 480 software version 1.5.0 (Roche Diagnostics, Indianapolis, Ind., USA).

    SYBR Green Assay:

    Article Title: RAAS inhibitors directly reduce diabetes‐induced renal fibrosis via growth factor inhibition
    Article Snippet: 500 ng Tgfb1, Pdgfb, Ctgf, Mmp2, Timp1, Timp2 mRNA from rat kidney samples and 500 ng TGFB1, PDGFB, and CTGF mRNA from human proximal tubular epithelial cells were reverse-transcribed using First Strand cDNA Synthesis Kit for RT-PCR (Thermo Fisher Scientific, Waltham, MA, USA). .. Aforementioned mRNA expressions were determined in triplicate of 1 μL cDNA sample by qPCR using 10 μL SYBR Green I Master enzyme mix (Roche Diagnostics, Mannheim, Germany) and 10 pmol μL -1 of each specific primers (Invitrogen, Budapest, Hungary) designed by Lasergene PrimerSelect software version 7.1.0 (DNASTAR, RRID:SCR_016295) based on nucleotide sequences from National Center for Biotechnology Information’s nucleotide database (primer sequences: Table 1). .. Results were analysed by LightCycler 480 software version 1.5.0 (Roche Diagnostics, This article is protected by copyright.

    Article Title: Use of Sigma-1 receptor agonist compounds
    Article Snippet: One hundred fifty nanograms each of FN1, COL1A1 and MMP2 mRNA from HTM-5 cells were reverse-transcribed using a First Strand cDNA Synthesis Kit for RT-PCR (Thermo Fisher Scientific, Waltham, Mass., USA). .. The expression of the mRNA was determined in triplicate with 1 μL cDNA samples obtained by qPCR using 10 μL SYBR Green I Master enzyme mix (Roche Diagnostics, Mannheim, Germany) and 10 pmol μL-1 of each specific primer (Invitrogen, Carlsbad, Calif., USA), following sequences designed by Lasergene PrimerSelect software version 7.1.0 (DNASTAR, Medison, Wis., USA) based on nucleotide sequences from National Center for Biotechnology Information's nucleotide database. .. Results were analysed by LightCycler 480 software version 1.5.0 (Roche Diagnostics, Indianapolis, Ind., USA).

    Expressing:

    Article Title: Use of Sigma-1 receptor agonist compounds
    Article Snippet: One hundred fifty nanograms each of FN1, COL1A1 and MMP2 mRNA from HTM-5 cells were reverse-transcribed using a First Strand cDNA Synthesis Kit for RT-PCR (Thermo Fisher Scientific, Waltham, Mass., USA). .. The expression of the mRNA was determined in triplicate with 1 μL cDNA samples obtained by qPCR using 10 μL SYBR Green I Master enzyme mix (Roche Diagnostics, Mannheim, Germany) and 10 pmol μL-1 of each specific primer (Invitrogen, Carlsbad, Calif., USA), following sequences designed by Lasergene PrimerSelect software version 7.1.0 (DNASTAR, Medison, Wis., USA) based on nucleotide sequences from National Center for Biotechnology Information's nucleotide database. .. Results were analysed by LightCycler 480 software version 1.5.0 (Roche Diagnostics, Indianapolis, Ind., USA).



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    99
    DNASTAR lasergene primerselect software version 7 1 0
    Lasergene Primerselect Software Version 7 1 0, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lasergene+primerselect+software+version+7+1+0/Lasergene/us10842794-621-46-47
    Average 99 stars, based on 1 article reviews
    lasergene primerselect software version 7 1 0 - by Bioz Stars, 2026-09
    99/100 stars
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